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housekeeping protein gapdh  (Bio-Rad)
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housekeeping protein antibodies include hrp conjugated rabbit anti gapdh  (Cell Signaling Technology Inc)
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housekeeping protein gapdh  (Cell Signaling Technology Inc)
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Housekeeping Protein Gapdh, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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housekeeping cytosolic protein gapdh  (Danaher Inc)
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A-D. Upper panels: Representative Western blots stained with ZO1 (220 kDa), OCLN (55kDa), CLDN5 (23 kDa), Actin (42 kDa) and calnexin (100 kDa) for bEnd.3 cells treated with vehicle (Ctrl) or exposed to 10 and 25 μM f1-C5C2. Lower panels: Quantification of protein expression normalized over the respective controls. Data are means ± sem (n = 4), *p < 0.05, **p < 0.01, one-way ANOVA/Friedman’s test. E. Representative confocal images for mCLDN5 immunostaining at various time points (2, 4, 6 and 8 h) for bEnd.3 cells treated with vehicle (Ctrl) or exposed to 10 and 25 μM f1-C5C2. The boxed areas are shown under each image at higher magnification. Scale bars, 25 μm. F. Results of the quantitative image analysis mCLDN5 subcellular distribution. Values of fluorescence intensity ratio of <t>cytosolic</t> and plasma membrane CLDN5 at various time points (2, 4, 6 and 8 h) were normalized over the respective controls. Data are means ± sem (n = 3), *p < 0.05, ** p < 0.01, ordinary one-way ANOVA test. G. Surface biotinylation of bEnd.3 cells treated with vehicle (Ctrl) or exposed to 25 μM f1-C5C2. Left panel: Representative blots stained with antibodies to mCLDN5 (18 kDa), the intracellular housekeeping protein <t>GAPDH</t> (36 kDa), and the plasma membrane-associated Na + /K + -ATPase 1 (NaK1; 130 KDa). Right panel: Quantification of mCLDN5 intracellular (cytosolic)/extracellular (membrane) expression normalized over the respective controls. Data are means ± sem (n = 3), *p < 0.05, unpaired Student’s t-test.
Housekeeping Cytosolic Protein Gapdh, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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housekeeping protein anti gapdh antibodies  (Santa Cruz Biotechnology)
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A-D. Upper panels: Representative Western blots stained with ZO1 (220 kDa), OCLN (55kDa), CLDN5 (23 kDa), Actin (42 kDa) and calnexin (100 kDa) for bEnd.3 cells treated with vehicle (Ctrl) or exposed to 10 and 25 μM f1-C5C2. Lower panels: Quantification of protein expression normalized over the respective controls. Data are means ± sem (n = 4), *p < 0.05, **p < 0.01, one-way ANOVA/Friedman’s test. E. Representative confocal images for mCLDN5 immunostaining at various time points (2, 4, 6 and 8 h) for bEnd.3 cells treated with vehicle (Ctrl) or exposed to 10 and 25 μM f1-C5C2. The boxed areas are shown under each image at higher magnification. Scale bars, 25 μm. F. Results of the quantitative image analysis mCLDN5 subcellular distribution. Values of fluorescence intensity ratio of <t>cytosolic</t> and plasma membrane CLDN5 at various time points (2, 4, 6 and 8 h) were normalized over the respective controls. Data are means ± sem (n = 3), *p < 0.05, ** p < 0.01, ordinary one-way ANOVA test. G. Surface biotinylation of bEnd.3 cells treated with vehicle (Ctrl) or exposed to 25 μM f1-C5C2. Left panel: Representative blots stained with antibodies to mCLDN5 (18 kDa), the intracellular housekeeping protein <t>GAPDH</t> (36 kDa), and the plasma membrane-associated Na + /K + -ATPase 1 (NaK1; 130 KDa). Right panel: Quantification of mCLDN5 intracellular (cytosolic)/extracellular (membrane) expression normalized over the respective controls. Data are means ± sem (n = 3), *p < 0.05, unpaired Student’s t-test.
Housekeeping Protein Anti Gapdh Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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housekeeping control protein anti gapdh antibody  (Danaher Inc)
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A-D. Upper panels: Representative Western blots stained with ZO1 (220 kDa), OCLN (55kDa), CLDN5 (23 kDa), Actin (42 kDa) and calnexin (100 kDa) for bEnd.3 cells treated with vehicle (Ctrl) or exposed to 10 and 25 μM f1-C5C2. Lower panels: Quantification of protein expression normalized over the respective controls. Data are means ± sem (n = 4), *p < 0.05, **p < 0.01, one-way ANOVA/Friedman’s test. E. Representative confocal images for mCLDN5 immunostaining at various time points (2, 4, 6 and 8 h) for bEnd.3 cells treated with vehicle (Ctrl) or exposed to 10 and 25 μM f1-C5C2. The boxed areas are shown under each image at higher magnification. Scale bars, 25 μm. F. Results of the quantitative image analysis mCLDN5 subcellular distribution. Values of fluorescence intensity ratio of <t>cytosolic</t> and plasma membrane CLDN5 at various time points (2, 4, 6 and 8 h) were normalized over the respective controls. Data are means ± sem (n = 3), *p < 0.05, ** p < 0.01, ordinary one-way ANOVA test. G. Surface biotinylation of bEnd.3 cells treated with vehicle (Ctrl) or exposed to 25 μM f1-C5C2. Left panel: Representative blots stained with antibodies to mCLDN5 (18 kDa), the intracellular housekeeping protein <t>GAPDH</t> (36 kDa), and the plasma membrane-associated Na + /K + -ATPase 1 (NaK1; 130 KDa). Right panel: Quantification of mCLDN5 intracellular (cytosolic)/extracellular (membrane) expression normalized over the respective controls. Data are means ± sem (n = 3), *p < 0.05, unpaired Student’s t-test.
Housekeeping Control Protein Anti Gapdh Antibody, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti glyceraldehyde 3 phosphate dehydrogenase housekeeping protein  (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc rabbit anti glyceraldehyde 3 phosphate dehydrogenase housekeeping protein
A-D. Upper panels: Representative Western blots stained with ZO1 (220 kDa), OCLN (55kDa), CLDN5 (23 kDa), Actin (42 kDa) and calnexin (100 kDa) for bEnd.3 cells treated with vehicle (Ctrl) or exposed to 10 and 25 μM f1-C5C2. Lower panels: Quantification of protein expression normalized over the respective controls. Data are means ± sem (n = 4), *p < 0.05, **p < 0.01, one-way ANOVA/Friedman’s test. E. Representative confocal images for mCLDN5 immunostaining at various time points (2, 4, 6 and 8 h) for bEnd.3 cells treated with vehicle (Ctrl) or exposed to 10 and 25 μM f1-C5C2. The boxed areas are shown under each image at higher magnification. Scale bars, 25 μm. F. Results of the quantitative image analysis mCLDN5 subcellular distribution. Values of fluorescence intensity ratio of <t>cytosolic</t> and plasma membrane CLDN5 at various time points (2, 4, 6 and 8 h) were normalized over the respective controls. Data are means ± sem (n = 3), *p < 0.05, ** p < 0.01, ordinary one-way ANOVA test. G. Surface biotinylation of bEnd.3 cells treated with vehicle (Ctrl) or exposed to 25 μM f1-C5C2. Left panel: Representative blots stained with antibodies to mCLDN5 (18 kDa), the intracellular housekeeping protein <t>GAPDH</t> (36 kDa), and the plasma membrane-associated Na + /K + -ATPase 1 (NaK1; 130 KDa). Right panel: Quantification of mCLDN5 intracellular (cytosolic)/extracellular (membrane) expression normalized over the respective controls. Data are means ± sem (n = 3), *p < 0.05, unpaired Student’s t-test.
Rabbit Anti Glyceraldehyde 3 Phosphate Dehydrogenase Housekeeping Protein, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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housekeeping protein gapdh  (Abcam)
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Abcam housekeeping protein gapdh
A-D. Upper panels: Representative Western blots stained with ZO1 (220 kDa), OCLN (55kDa), CLDN5 (23 kDa), Actin (42 kDa) and calnexin (100 kDa) for bEnd.3 cells treated with vehicle (Ctrl) or exposed to 10 and 25 μM f1-C5C2. Lower panels: Quantification of protein expression normalized over the respective controls. Data are means ± sem (n = 4), *p < 0.05, **p < 0.01, one-way ANOVA/Friedman’s test. E. Representative confocal images for mCLDN5 immunostaining at various time points (2, 4, 6 and 8 h) for bEnd.3 cells treated with vehicle (Ctrl) or exposed to 10 and 25 μM f1-C5C2. The boxed areas are shown under each image at higher magnification. Scale bars, 25 μm. F. Results of the quantitative image analysis mCLDN5 subcellular distribution. Values of fluorescence intensity ratio of <t>cytosolic</t> and plasma membrane CLDN5 at various time points (2, 4, 6 and 8 h) were normalized over the respective controls. Data are means ± sem (n = 3), *p < 0.05, ** p < 0.01, ordinary one-way ANOVA test. G. Surface biotinylation of bEnd.3 cells treated with vehicle (Ctrl) or exposed to 25 μM f1-C5C2. Left panel: Representative blots stained with antibodies to mCLDN5 (18 kDa), the intracellular housekeeping protein <t>GAPDH</t> (36 kDa), and the plasma membrane-associated Na + /K + -ATPase 1 (NaK1; 130 KDa). Right panel: Quantification of mCLDN5 intracellular (cytosolic)/extracellular (membrane) expression normalized over the respective controls. Data are means ± sem (n = 3), *p < 0.05, unpaired Student’s t-test.
Housekeeping Protein Gapdh, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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A-D. Upper panels: Representative Western blots stained with ZO1 (220 kDa), OCLN (55kDa), CLDN5 (23 kDa), Actin (42 kDa) and calnexin (100 kDa) for bEnd.3 cells treated with vehicle (Ctrl) or exposed to 10 and 25 μM f1-C5C2. Lower panels: Quantification of protein expression normalized over the respective controls. Data are means ± sem (n = 4), *p < 0.05, **p < 0.01, one-way ANOVA/Friedman’s test. E. Representative confocal images for mCLDN5 immunostaining at various time points (2, 4, 6 and 8 h) for bEnd.3 cells treated with vehicle (Ctrl) or exposed to 10 and 25 μM f1-C5C2. The boxed areas are shown under each image at higher magnification. Scale bars, 25 μm. F. Results of the quantitative image analysis mCLDN5 subcellular distribution. Values of fluorescence intensity ratio of cytosolic and plasma membrane CLDN5 at various time points (2, 4, 6 and 8 h) were normalized over the respective controls. Data are means ± sem (n = 3), *p < 0.05, ** p < 0.01, ordinary one-way ANOVA test. G. Surface biotinylation of bEnd.3 cells treated with vehicle (Ctrl) or exposed to 25 μM f1-C5C2. Left panel: Representative blots stained with antibodies to mCLDN5 (18 kDa), the intracellular housekeeping protein GAPDH (36 kDa), and the plasma membrane-associated Na + /K + -ATPase 1 (NaK1; 130 KDa). Right panel: Quantification of mCLDN5 intracellular (cytosolic)/extracellular (membrane) expression normalized over the respective controls. Data are means ± sem (n = 3), *p < 0.05, unpaired Student’s t-test.

Journal: bioRxiv

Article Title: A claudin5-binding peptide enhances the permeability of the blood-brain-barrier

doi: 10.1101/2024.04.29.591687

Figure Lengend Snippet: A-D. Upper panels: Representative Western blots stained with ZO1 (220 kDa), OCLN (55kDa), CLDN5 (23 kDa), Actin (42 kDa) and calnexin (100 kDa) for bEnd.3 cells treated with vehicle (Ctrl) or exposed to 10 and 25 μM f1-C5C2. Lower panels: Quantification of protein expression normalized over the respective controls. Data are means ± sem (n = 4), *p < 0.05, **p < 0.01, one-way ANOVA/Friedman’s test. E. Representative confocal images for mCLDN5 immunostaining at various time points (2, 4, 6 and 8 h) for bEnd.3 cells treated with vehicle (Ctrl) or exposed to 10 and 25 μM f1-C5C2. The boxed areas are shown under each image at higher magnification. Scale bars, 25 μm. F. Results of the quantitative image analysis mCLDN5 subcellular distribution. Values of fluorescence intensity ratio of cytosolic and plasma membrane CLDN5 at various time points (2, 4, 6 and 8 h) were normalized over the respective controls. Data are means ± sem (n = 3), *p < 0.05, ** p < 0.01, ordinary one-way ANOVA test. G. Surface biotinylation of bEnd.3 cells treated with vehicle (Ctrl) or exposed to 25 μM f1-C5C2. Left panel: Representative blots stained with antibodies to mCLDN5 (18 kDa), the intracellular housekeeping protein GAPDH (36 kDa), and the plasma membrane-associated Na + /K + -ATPase 1 (NaK1; 130 KDa). Right panel: Quantification of mCLDN5 intracellular (cytosolic)/extracellular (membrane) expression normalized over the respective controls. Data are means ± sem (n = 3), *p < 0.05, unpaired Student’s t-test.

Article Snippet: Membranes were then incubated overnight with antibodies against CLDN5 (rabbit polyclonal, Invitrogen, #PA599415, 1:500) and the housekeeping cytosolic protein GAPDH (rabbit polyclonal, Abcam 9485, 1:5000) and the plasma membrane protein NaK1 (mouse monoclonal, Millipore, 05-369, 1:2000).

Techniques: Western Blot, Staining, Expressing, Immunostaining, Fluorescence, Membrane